The Rat Collagen Type IV Alpha 1 (COL4A1) ELISA Kit from Assay Genie is specifically designed for the accurate and reliable detection of COL4A1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with precise and reproducible results for a variety of research applications.Collagen Type IV Alpha 1 is a key component of the basement membrane and is essential for maintaining tissue structure and function. Abnormalities in COL4A1 expression have been linked to various diseases and conditions, such as kidney disease, muscular dystrophy, and certain cancers.
Therefore, the Rat COL4A1 ELISA Kit serves as a valuable tool for investigating the role of COL4A1 in these conditions and exploring potential therapeutic interventions.With its advanced technology and user-friendly format, the Rat COL4A1 ELISA Kit is an indispensable resource for researchers studying collagen-related processes and diseases in rat models. Trust in Assay Genie's expertise to deliver accurate and actionable results for your research needs.
Product Name:
Rat Collagen Type IV Alpha 1 (COL4a1) ELISA Kit
Product Code:
RTDL00232
Size:
96 Assays
Target:
COL4a1
Synonyms:
COL4-A1, Arresten, Collagen Alpha-1(IV)chain
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
