Rat COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit
The Rat COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit is specifically designed for the quantitative detection of COMP levels in rat biological samples. COMP is a key structural component of the extracellular matrix in cartilage and other tissues, playing a critical role in maintaining tissue integrity and function. Quantifying COMP levels is crucial for investigating cartilage health, joint diseases, and musculoskeletal disorders. Our ELISA kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Manufactained under strict quality control measures, this kit offers robust performance and user-friendly protocols, making it a valuable tool for studying cartilage biology, disease mechanisms, and therapeutic interventions.
Product Name:
Rat COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit
SKU:
AEES00343
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
