SynonymsASP,CPAMD1,Complement Protein C3,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1,C3a anaphylatoxin,Acylation stimulating protein
ReactivityRat
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
RTDL00158
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01026
Sensitivity:
10.62ng/mL
Range:
31.25-2000ng/mL
ELISA Type:
Sandwich
Synonyms:
ASP,CPAMD1,Complement Protein C3,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1,C3a anaphylatoxin,Acylation stimulating protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Rat Complement Component 3 (C3) ELISA Kit is specifically designed for the precise measurement of C3 levels in rat serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.C3 is an essential protein in the complement system, playing a key role in immune response and inflammation. Dysregulation of C3 has been linked to various inflammatory diseases, autoimmune disorders, and infectious conditions, making it a valuable biomarker for studying these diseases and exploring therapeutic interventions.
With its reliable performance and ease of use, the Rat C3 ELISA Kit is a valuable tool for researchers seeking to investigate the role of C3 in disease pathogenesis and explore potential therapeutic targets.
Product Name:
Rat Complement Component 3 (C3) ELISA Kit
Product Code:
RTDL00158
Size:
96 Assays
Target:
C3
Synonyms:
ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein
Detection Method:
Sandwich
Range:
31.25-2000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
