Rat Corticotropin Releasing Hormone Binding Protein (CRHBP) ELISA Kit
The Rat Corticotropin-Releasing Hormone Binding Protein (CRHBP) ELISA Kit is a specialized assay designed for the precise measurement of CRHBP levels in rat biological samples, including serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit provides accurate and consistent results, making it an invaluable tool for a variety of research applications.CRHBP is a key protein involved in the regulation of stress responses and the hypothalamic-pituitary-adrenal axis. It binds to corticotropin-releasing hormone (CRH) and modulates its activity, playing a crucial role in the neuroendocrine system.
By measuring CRHBP levels, researchers can gain insights into various physiological and pathological processes related to stress, mood disorders, and neuroendocrine dysregulation.Whether studying the impact of stress on rat models or investigating potential therapeutic interventions for stress-related disorders, the Rat CRHBP ELISA Kit offers reliable and reproducible results, helping to advance our understanding of CRHBP biology and its implications for human health.
Product Name:
Rat Corticotropin Releasing Hormone Binding Protein (CRHBP) ELISA Kit
Product Code:
RTDL00250
Size:
96 Assays
Target:
CRHBP
Synonyms:
CRF-BP, CRFBP, Corticotropin-Releasing Factor-Binding Protein
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
