The Rat CXCR4 (C-X-C Motif Receptor 4) ELISA Kit is a powerful tool for detecting levels of CXCR4 in rat samples with high accuracy and precision. This kit is specifically designed for use with rat serum, plasma, and tissue homogenates, providing researchers with a reliable method for studying the expression of CXCR4 in various biological samples. CXCR4 is a key chemokine receptor that plays a critical role in processes such as immune cell migration, hematopoiesis, and stem cell homing. Dysregulation of CXCR4 has been implicated in various diseases, including cancer, HIV/AIDS, and inflammatory disorders.
Therefore, this ELISA kit offers researchers a valuable tool for investigating the role of CXCR4 in disease pathogenesis and developing potential therapeutic interventions. With its high sensitivity and specificity, the Rat CXCR4 ELISA Kit delivers consistent and reproducible results, making it an essential tool for researchers working in the fields of immunology, oncology, and stem cell biology. Invest in this kit today to enhance your research and gain a better understanding of CXCR4 biology in rat models.
Product Name:
Rat CXCR4 (C-X-C motif receptor 4) ELISA Kit
SKU:
RTFI01297
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.039 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-93%
83-97%
85-99%
EDTA Plasma (n = 5)
99-104%
82-96%
89-96%
Heparin Plasma (n = 5)
91-103%
82-96%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-105
92
EDTA Plasma (n = 5)
89-105
99
Heparin Plasma (n = 5)
91-105
100
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
