Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit
The Rat Cyclin-Dependent Kinase Inhibitor 1a (CDKN1a) ELISA Kit is a cutting-edge assay specifically designed for the precise measurement of CDKN1a levels in rat samples, including serum, plasma, and tissue homogenates. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for a variety of research applications.CDKN1a, also known as p21, is a key regulator of cell cycle progression and cell growth, functioning as a tumor suppressor by inhibiting cyclin-dependent kinases.
Dysregulation of CDKN1a has been implicated in various diseases, including cancer and neurodegenerative disorders, underscoring its importance as a potential therapeutic target and diagnostic marker. By utilizing the Rat CDKN1a ELISA Kit, researchers can gain valuable insights into the role of CDKN1a in disease pathogenesis and explore novel treatment strategies.
Product Name:
Rat Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit
Product Code:
RTDL00197
Size:
96 Assays
Target:
CDKN1A
Synonyms:
WAF1, p21, CIP1, p21WAF1, CAP20, CDKN1, MDA-6, SDI1, p21CIP1, CDK-Interacting Protein 1, Melanoma differentiation-associated protein 6
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
