The Rat Fetuin-B (FetuB) ELISA Kit is a powerful tool for the precise measurement of rat Fetuin-B levels in various biological samples such as serum, plasma, and tissue homogenates. This kit offers exceptional accuracy and specificity, ensuring dependable and consistent results for a diverse array of research experiments.Fetuin-B, also known as FetuB, is a key protein involved in regulating metabolic processes and insulin sensitivity. It plays a significant role in various physiological and pathological conditions, including obesity, diabetes, and liver diseases.
By accurately quantifying Fetuin-B levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets.The Rat Fetuin-B (FetuB) ELISA Kit is a valuable asset for scientists studying metabolic disorders, inflammation, and endocrine regulation. Its high-quality components and user-friendly protocol make it a reliable choice for research laboratories seeking precise and reproducible data on rat Fetuin-B expression levels.
Product Name:
Rat Fetuin B (FETUB) ELISA Kit
Product Code:
RTDL00368
Size:
96 Assays
Target:
FETUB
Synonyms:
FETU-B, 16G2, Gugu, IRL685, Fetuin-like protein IRL685
Detection Method:
Sandwich
Range:
31.25-2000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
