The Rat Galectin-7 (Gal7) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of Galectin-7 in rat samples. This kit is highly sensitive and specific, providing reliable and consistent results for a variety of research applications.Galectin-7 is a protein known for its involvement in processes such as cell adhesion, apoptosis, and regulation of immune responses. Research has shown that dysregulation of Galectin-7 expression is associated with various diseases, including cancer and inflammatory disorders, highlighting its importance as a potential biomarker.
With the Rat Galectin-7 CLIA Kit, researchers can gain valuable insights into the role of Galectin-7 in disease progression and development, paving the way for new therapeutic strategies and interventions.
Product Name:
Rat GAL7 (Galectin 7) CLIA Kit
SKU:
RTES00226
Target:
Rat GAL7 (Galectin 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL7. You can calculate the concentration of Rat GAL7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
87-100
98-113
Average (%)
104
94
106
1:4
Range (%)
102-119
87-99
97-108
Average (%)
109
93
102
1:8
Range (%)
100-117
97-111
103-118
Average (%)
108
104
109
1:16
Range (%)
87-100
84-97
87-99
Average (%)
94
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
99-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.49
316.64
685.93
93.11
335.81
631.04
Standard deviation
10.48
22.8
50.07
10.82
34.69
68.22
C V (%)
11.45
7.2
7.3
11.62
10.33
10.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL7 in samples. No significant cross-reactivity or interference between Rat GAL7 and analogues was observed.
