Rat Galactose-1-Phosphate Uridylyltransferase (GALT) ELISA Kit
The Rat Galactose-1-Phosphate Uridylyltransferase (GALT) ELISA Kit is specifically designed for the quantitative detection of rat GALT levels in biological samples such as serum, plasma, and tissue homogenates. With high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.GALT is an important enzyme involved in galactose metabolism, playing a crucial role in the breakdown of galactose-1-phosphate. Dysregulation of GALT activity is associated with galactosemia, a rare genetic disorder that can lead to severe health complications if left untreated.
Therefore, monitoring GALT levels can be valuable for studying this condition and developing potential therapeutic interventions.This ELISA kit offers a convenient and efficient way to measure GALT levels, allowing researchers to explore the role of this enzyme in various physiological and pathological processes. Whether studying galactose metabolism or investigating the impact of GALT dysregulation in disease states, the Rat GALT ELISA Kit provides a reliable tool for accurate quantification of GALT levels in rat samples.
Product Name:
Rat Galactose-1-Phosphate Uridylyltransferase (GALT) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
