Rat Gamma-Secretase Activating Protein (gSAP) ELISA Kit
The Rat GSAP (Gamma Secretase Activating Protein) ELISA Kit is specifically designed for the accurate measurement of GSAP levels in rat samples, including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and dependable results for researchers in various fields.GSAP is a key regulator of gamma-secretase activity, a crucial enzyme involved in the processing of amyloid precursor protein (APP) and the generation of amyloid beta peptides.
Dysregulation of GSAP has been linked to Alzheimer's disease and other neurodegenerative disorders, making it a valuable target for drug development and biomarker studies.With its reliable performance and ease of use, the Rat GSAP ELISA Kit is an essential tool for investigating the role of GSAP in disease pathology and exploring potential therapeutic interventions.
Product Name:
Rat Gamma-Secretase Activating Protein (gSAP) ELISA Kit
Product Code:
RTDL00464
Size:
96 Assays
Target:
gSAP
Synonyms:
PION, Pigeon Homolog
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
