The Rat Ghrelin ELISA Kit is specifically designed for the precise measurement of ghrelin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing trustworthy and consistent results for various research purposes. Ghrelin is a vital hormone known to regulate appetite, energy metabolism, and growth hormone release. It plays a crucial role in conditions such as obesity, diabetes, and gastrointestinal disorders, making it a valuable biomarker for studying these diseases and exploring therapeutic interventions. Overall, the Rat Ghrelin ELISA Kit is a valuable tool for researchers seeking to investigate the role of ghrelin in physiological processes and disease states, providing accurate and reliable data for further analysis and interpretation.
Product Name:
Rat GHRL (Ghrelin) ELISA Kit
SKU:
RTES00707
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
