Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit
The Rat Glycogen Synthase Kinase-3 Alpha (GSK3A) ELISA Kit is specifically designed for the accurate measurement of GSK3A levels in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.GSK3A is a key enzyme involved in various cellular processes, including glycogen metabolism, cell cycle regulation, and gene transcription. Dysregulation of GSK3A has been implicated in a range of diseases, including cancer, diabetes, and neurodegenerative disorders.
As such, measuring GSK3A levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions. Overall, the Rat Glycogen Synthase Kinase-3 Alpha (GSK3A) ELISA Kit is a valuable tool for researchers studying the role of GSK3A in disease pathogenesis and exploring its potential as a therapeutic target.
Product Name:
Rat Glycogen Synthase Kinase 3 Alpha (GSK3a) ELISA Kit
Product Code:
RTDL00465
Size:
96 Assays
Target:
GSK3a
Synonyms:
Serine/threonine-protein kinase GSK3A
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
