The Rat Glycoprotein A33 (GPA33) ELISA Kit from AssayGenie is a reliable and sensitive assay designed for the accurate quantification of GPA33 levels in rat serum, plasma, and tissue lysates. This kit provides precise and reproducible results, making it a valuable tool for researchers in a variety of fields.GPA33 is a glycoprotein that plays a key role in cell adhesion and may be a potential target for cancer therapy. It is commonly overexpressed in colorectal cancer and has been implicated in tumor progression and metastasis.
By measuring GPA33 levels, researchers can gain valuable insights into the role of this protein in cancer biology and potentially identify new therapeutic targets.Overall, the Rat GPA33 ELISA Kit from AssayGenie offers a reliable and efficient solution for studying the role of GPA33 in cancer and other diseases. Its high sensitivity and specificity make it an ideal tool for research labs looking to expand their understanding of this important glycoprotein.
Product Name:
Rat Glycoprotein A33 (GPA33) ELISA Kit
Product Code:
RTDL00450
Size:
96 Assays
Target:
GPA33
Synonyms:
Cell surface A33 antigen
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
