The Rat GREM1 (Gremlin-1) CLIA Kit is specifically designed for the precise measurement of GREM1 levels in rat serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.GREM1, also known as Gremlin-1, is a key protein involved in regulating developmental processes and cell growth. It plays a critical role in various diseases such as cancer, cardiovascular disorders, and fibrosis, making it a valuable biomarker for studying these conditions and exploring potential treatments.
With the Rat GREM1 CLIA Kit, researchers can uncover valuable insights into the role of GREM1 in disease progression and potentially identify novel therapeutic targets for targeted interventions. Get your kit today and advance your research in the field of GREM1 biology.
Product Name:
Rat GREM1 (GremLin 1) CLIA Kit
SKU:
RTES00263
Target:
Rat GREM1 (GremLin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GREM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GREM1. You can calculate the concentration of Rat GREM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
91-104
91-105
Average (%)
96
97
99
1:4
Range (%)
93-107
100-116
92-107
Average (%)
99
107
98
1:8
Range (%)
90-103
90-101
94-108
Average (%)
95
96
99
1:16
Range (%)
102-118
86-98
88-104
Average (%)
110
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.21
304.79
839.31
116.85
294.21
776.23
Standard deviation
12.64
21.67
66.73
15.18
32.69
54.49
C V (%)
11.68
7.11
7.95
12.99
11.11
7.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GREM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GREM1 in samples. No significant cross-reactivity or interference between Rat GREM1 and analogues was observed.
