The Rat Hemojuvelin (HJV) ELISA Kit is a highly sensitive and specific assay designed for the precise quantification of HJV levels in rat serum, plasma, and cell culture supernatants. This kit allows for accurate and reproducible results, making it ideal for various research applications.Hemojuvelin is a critical protein involved in iron metabolism and regulation, playing a crucial role in conditions such as iron overload disorders and anemia.
By measuring HJV levels, researchers can gain valuable insights into the mechanisms underlying these disorders and develop potential therapeutic strategies.Overall, the Rat Hemojuvelin (HJV) ELISA Kit is a powerful tool for studying the role of HJV in iron homeostasis and related disorders, offering researchers a reliable and efficient method for quantifying HJV levels in rat samples.
Product Name:
Rat Hemojuvelin (HJV) ELISA Kit
Product Code:
RTDL00487
Size:
96 Assays
Target:
HJV
Synonyms:
HFE2, HFE2A, HJV, JH, RGMC, hemochromatosis type 2(Juvenile), Haemojuvelin, RGM domain family member C
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
