The Rat I-PTH (Intact Parathormone) ELISA Kit is a specialized assay designed for the quantitative measurement of Intact Parathormone levels in a variety of biological samples. Intact Parathormone, a crucial hormone produced by the parathyroid glands, plays a significant role in calcium and phosphorus homeostasis, bone metabolism, and renal function regulation. Accurate quantification of I-PTH is essential for understanding its pivotal role in maintaining mineral balance and bone health. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly operation, making it a valuable tool for in-depth research applications.
Product Name:
Rat I-PTH (Intact Parathormone) ELISA Kit
SKU:
AEES00445
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
