The Rat IL-1β ELISA Kit is a specialized assay designed to quantitatively measure the levels of Interleukin-1 beta (IL-1β) in various rat biological samples. IL-1β is a pivotal pro-inflammatory cytokine involved in a diverse range of cellular functions, such as cell proliferation, differentiation, and apoptosis. Its role in mediating inflammatory reactions is crucial in the body's immune response and contributes to various inflammatory diseases, autoimmune conditions, and infections. Accurate measurement of IL-1β levels with this ELISA kit is essential for gaining insights into the mechanisms of disease pathology and developing precise therapeutic interventions.
This ELISA kit provided by Assay Genie ensures exceptional sensitivity and specificity, leading to reliable and reproducible outcomes. Manufactured under strict quality control measures, the IL-1β ELISA Kit delivers robust performance and user-friendly operation, making it an ideal tool for researchers aiming to investigate the functions and impact of IL-1β in biological systems.
Product Name:
Rat IL-1beta ELISA Kit
SKU:
AEES00294
Target:
Rat IL-1β
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
86-96
100-116
Average (%)
92
91
106
1:4
Range (%)
95-105
84-96
94-106
Average (%)
100
91
100
1:8
Range (%)
101-114
81-91
96-112
Average (%)
108
86
103
1:16
Range (%)
96-108
83-97
92-106
Average (%)
102
89
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.5
314.2
880.1
107.4
340.6
954.8
Standard deviation
5.7
13.5
30.8
5.6
20.4
33.4
C V (%)
5.67
4.3
3.5
5.21
5.99
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1β in samples. No significant cross-reactivity or interference between Rat IL-1β and analogues was observed.
