The Rat IL-1β ELISA Kit is a specialized assay designed to quantitatively measure the levels of Interleukin-1 beta (IL-1β) in various rat biological samples. IL-1β is a pivotal pro-inflammatory cytokine involved in a diverse range of cellular functions, such as cell proliferation, differentiation, and apoptosis. Its role in mediating inflammatory reactions is crucial in the body's immune response and contributes to various inflammatory diseases, autoimmune conditions, and infections. Accurate measurement of IL-1β levels with this ELISA kit is essential for gaining insights into the mechanisms of disease pathology and developing precise therapeutic interventions. This ELISA kit provided by Assay Genie ensures exceptional sensitivity and specificity, leading to reliable and reproducible outcomes. Manufactured under strict quality control measures, the IL-1β ELISA Kit delivers robust performance and user-friendly operation, making it an ideal tool for researchers aiming to investigate the functions and impact of IL-1β in biological systems.
Product Name:
Rat IL-1beta ELISA Kit
SKU:
AEES00294
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
