The Rat IL-10 ELISA Kit is meticulously designed to facilitate the precise quantitative assessment of Interleukin-10 (IL-10) levels within a diverse range of rat biological samples. IL-10, a key immunoregulatory cytokine, plays a pivotal role in modulating both innate and adaptive immune responses, contributing significantly to immune tolerance and anti-inflammatory processes. Comprehensive measurement of IL-10 levels is crucial for elucidating its immunomodulatory functions and its involvement in various immune-related pathways, autoimmune conditions, and infectious diseases. Our IL-10 ELISA Kit from Assay Genie ensures unparalleled sensitivity and specificity, guaranteeing accurate and reproducible outcomes. Crafted with rigorous quality standards, this kit promises consistent and robust performance, coupled with user-friendly procedures, making it an optimal choice for researchers seeking to explore the intricate roles of IL-10 in diverse research settings.
Product Name:
Rat IL-10 ELISA Kit
SKU:
AEES00298
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
