The Rat IL-17A ELISA Kit is meticulously designed to facilitate the precise and quantitative determination of Interleukin-17A (IL-17A) levels in diverse rat biological samples. IL-17A serves as a pivotal pro-inflammatory cytokine, playing a crucial role in orchestrating immune responses, inflammation, and defense mechanisms. Its involvement spans a spectrum of immune-related functions, contributing significantly to the regulation of inflammatory cascades and immune cell activation. Designed by Assay Genie, the IL-17A ELISA Kit ensures unparalleled sensitivity and specificity, guaranteeing the generation of accurate and reproducible results. With a commitment to stringent quality control measures, this kit delivers robust performance and user-friendly operation, rendering it an indispensable tool for researchers aiming to unravel the complex roles of IL-17A in various physiological and pathological processes.
Product Name:
Rat IL-17A ELISA Kit
SKU:
AEES00457
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
