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Rat IL-6 PharmaGenie ELISA Kit (SBRS1665)

SKU:
SBRS1665
Product type:
ELISA Kit
Reactivity:
Rat
Application:
ELISA
ELISA Type:
Sandwich
€635
Frequently bought together:

Description

system_update_altDatasheet

Rat IL-6 PharmaGenie ELISA - Kit Data

Product Sample Types Method
Rat IL-6 PharmaGenie ELISA Kit (SBRS1664)Cell Culture Supernatants, Plasma and SerumSandwich
Rat IL-6 PharmaGenie ELISA Kit (SBRS1665)Cell and Tissue LysatesSandwich
Product SKU:SBRS1665
Size:96T
Application:This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Uniprot:P20607
Gene ID:24498
Gene Names:IL-6 / IL6
Synonyms:Interleukin-6 (IL-6)
Target Species:Rat
Compatible Sample Types:Cell and Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:30 pg/ml - 10000 pg/ml
Sensitivity:30 pg/ml
Recommended Dilution:2 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Stop Solution
4.Assay Diluent(s)
5.Lyophilized Standard
6.Biotinylated Detection Antibody
7.Streptavidin-Conjugated HRP
8.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Rat IL-6 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 ul of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 ul of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.

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