The Rat IL-1 Beta CLIA Kit (RTES00012) from Assay Genie is a powerful tool for measuring IL-1 Beta levels in rat samples. This kit offers high sensitivity and specificity, providing researchers with accurate and reliable results for their experiments.IL-1 Beta is a key pro-inflammatory cytokine involved in the body's immune response, playing a crucial role in various inflammatory and autoimmune diseases. By detecting IL-1 Beta levels, researchers can gain valuable insights into the mechanisms underlying these conditions and identify potential therapeutic targets.
With its easy-to-use format and quick assay time, the Rat IL-1 Beta CLIA Kit is suitable for a wide range of applications in biomedical research. Whether studying inflammation, autoimmune disorders, or drug development, this kit is a valuable tool for advancing scientific discovery.
Product Name:
Rat IL-1 beta CLIA Kit
SKU:
RTES00012
Target:
Rat IL-1 beta
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
97-114
96-109
Average (%)
109
104
101
1:4
Range (%)
97-113
87-100
97-113
Average (%)
103
93
104
1:8
Range (%)
95-110
94-105
87-101
Average (%)
101
100
92
1:16
Range (%)
101-112
98-113
97-112
Average (%)
107
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.49
83.36
301.9
38.3
77.17
276.82
Standard deviation
3.89
8.99
30.1
4.29
8.21
22.84
C V (%)
10.66
10.78
9.97
11.2
10.64
8.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1β in samples. No significant cross-reactivity or interference between Rat IL-1β and analogues was observed.
