The Rat INS (Insulin) ELISA Kit is a precision-designed assay developed for the quantitative detection and measurement of insulin levels in rat samples. Insulin is a critical hormone involved in regulating blood glucose levels, carbohydrate metabolism, and energy balance. This ELISA kit provides researchers with a reliable method to quantify insulin concentrations accurately, facilitating studies related to diabetes, metabolism, and endocrine function. Accurate measurement of insulin levels using this ELISA kit is vital for investigating various physiological and pathological processes, including glucose homeostasis, insulin resistance, and diabetes mellitus. By enabling precise quantification of insulin, researchers can gain insights into the mechanisms underlying metabolic disorders and explore potential therapeutic strategies aimed at managing diabetes and related conditions. The Rat INS ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing dependable and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly operation, making it an ideal choice for researchers conducting studies on insulin and its role in metabolic regulation and disease states.
Product Name:
Rat INS (Insulin) ELISA Kit
SKU:
AEES00513
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
