Rat Insulin PharmaGenie ELISA Kit (SBRS1668)
- SKU:
- SBRS1668
- Product type:
- ELISA Kit
- Reactivity:
- Rat
- Application:
- ELISA
- ELISA Type:
- Sandwich
Frequently bought together:
Description
system_update_altDatasheet
Rat Insulin PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS1668 |
| Size: | 96T |
| Application: | This ELISA antibody pair detects rat, mouse, human, porcine and bovine Insulin and Proinsulin. |
| Uniprot: | P01323 |
| Gene ID: | 24506 |
| Gene Names: | INS-2 / INS2 |
| Synonyms: | Insulin-1 [Cleaved into: Insulin-1 B chain Insulin-1 A chain] |
| Target Species: | Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 5 uIU/ml - 300 uIU/mL |
| Sensitivity: | 5 uIU/mL |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Stop Solution |
| 4. | Assay Diluent(s) |
| 5. | Lyophilized Standard |
| 6. | Biotinylated Detection Antibody |
| 7. | Streptavidin-Conjugated HRP |
| 8. | TMB One-Step Substrate |
Other materials and equipment required:
The Assay Genie Rat Insulin ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Kit Protocol:
| 1. | Prepare all reagents, samples and standards as instructed in the manual. |
| 2. | Add 100 ul of standard or sample to each well. |
| 3. | Incubate 2.5 h at RT or O/N at 4°C. |
| 4. | Add 100 ul of prepared biotin antibody to each well. |
| 5. | Incubate 1 h at RT. |
| 6. | Add 100 ul of prepared Streptavidin solution to each well. |
| 7. | Incubate 45 min at RT. |
| 8. | Add 100 ul of TMB One-Step Substrate Reagent to each well. |
| 9. | Incubate 30 min at RT. |
| 10. | Add 50 ul of Stop Solution to each well. |
| 11. | Read at 450 nm immediately. |
Related Products
