Rat Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA Kit
The Rat Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) ELISA Kit is specially designed for the accurate detection of IRAK4 levels in rat serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications. IRAK4 is a key enzyme in the Toll-like receptor (TLR) signaling pathway, playing a critical role in the regulation of the immune response. Dysregulation of IRAK4 has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.
Whether you are investigating the role of IRAK4 in immune responses, inflammation, or autoimmune diseases, the Rat Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) ELISA Kit provides the tools you need for accurate and precise quantification of IRAK4 levels in rat samples.
Product Name:
Rat Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA Kit
Product Code:
RTDL00584
Size:
96 Assays
Target:
IRAK4
Synonyms:
NY-REN-64
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
