The Rat Interleukin-5 (IL-5) ELISA Kit is a powerful tool for the precise measurement of IL-5 levels in rat serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for various research experiments.IL-5 is an important cytokine that plays a key role in regulating the immune response, particularly in the development and function of eosinophils. Dysregulation of IL-5 expression has been linked to various allergic and inflammatory diseases, making it a critical target for therapeutic interventions.
Whether you are studying asthma, allergies, or other immune-mediated disorders, the Rat IL-5 ELISA Kit provides a reliable means to quantify IL-5 levels and gain valuable insights into the underlying mechanisms of these conditions. Trust in this kit to enhance your research and move one step closer to novel treatment strategies.
Product Name:
Rat Interleukin 5 (IL-5) ELISA Kit
Product Code:
RTDL00567
Size:
96 Assays
Target:
IL5
Synonyms:
E-CSF, ESGF, EDF, TRF, Colony-Stimulating Factor,Eosinophil, T-Cell replacing Factor, B Cell Differentiation Factor I, Eosinophil Differentiation Factor
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
