The Rat IRE1 (Inositol-Requiring Enzyme 1) ELISA Kit is a reliable and accurate tool for detecting levels of IRE1 in rat samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and precise results, making it perfect for various research applications. IRE1 is a key enzyme in the unfolded protein response pathway, playing a crucial role in maintaining cellular homeostasis and response to stress.
Dysregulation of IRE1 has been implicated in various diseases including cancer, diabetes, and neurodegenerative disorders, highlighting the importance of studying this enzyme as a potential therapeutic target. With the Rat IRE1 ELISA Kit, researchers can confidently analyze IRE1 levels in rat samples to further understand its role in disease pathogenesis and potential treatment strategies.
Product Name:
Rat IRE1 (Inositol requiring enzyme 1) ELISA Kit
SKU:
RTFI01384
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
28.125 pg/mL
Range:
46.875-3000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
93-104%
87-105%
94-105%
EDTA Plasma (n = 5)
88-99%
89-96%
82-96%
Heparin Plasma (n = 5)
84-99%
83-98%
82-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-103
92
EDTA Plasma (n = 5)
86-100
91
Heparin Plasma (n = 5)
87-103
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
