Rat LCAT/Lecithin-cholesterol acyltransferase ELISA Kit
The Rat LCAT (Lecithin-Cholesterol Acyltransferase) ELISA Kit is specially designed for the accurate quantification of LCAT levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research purposes. LCAT is an important enzyme involved in lipid metabolism, specifically in the conversion of free cholesterol to cholesteryl esters, playing a crucial role in the maintenance of cholesterol homeostasis.
Dysregulation of LCAT activity has been implicated in several cardiovascular diseases, making it a valuable marker for studying these conditions and exploring potential therapeutic interventions. With easy-to-follow protocols and reliable performance, the Rat LCAT ELISA Kit is a valuable tool for researchers investigating lipid metabolism, cardiovascular health, and related diseases in rat models. Get accurate and reproducible results with this kit from AssayGenie.
Product Name:
Rat LCAT/Lecithin-cholesterol acyltransferase ELISA Kit
SKU:
RTFI00925
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.375 ng/mL
Range:
0.625-40 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-101%
85-97%
81-99%
EDTA Plasma (n = 5)
90-99%
85-101%
83-94%
Heparin Plasma (n = 5)
87-102%
82-95%
91-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
94
EDTA Plasma (n = 5)
92-98
96
Heparin Plasma (n = 5)
85-100
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
