Rat Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) ELISA Kit
The Rat Lymphocyte Function-Associated Antigen 1 Alpha (LFA1A) ELISA Kit is a reliable tool for measuring levels of LFA1A in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.LFA1A is a critical protein involved in lymphocyte function, playing a key role in immune responses and cell-cell interactions. Dysregulation of LFA1A has been associated with autoimmune diseases, inflammatory conditions, and immune deficiencies, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.
Overall, the Rat LFA1A ELISA Kit offers researchers a valuable tool for investigating the role of LFA1A in immune system function and disease pathogenesis, ultimately paving the way for new insights and potential treatment strategies.
Product Name:
Rat Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) ELISA Kit
Product Code:
RTDL00629
Size:
96 Assays
Target:
LFA1a
Synonyms:
CD11a, ITG-AL, LFA-1, LFA1-A, ITGAL, Integrin,Alpha L, CD11 antigen-like family member A, Leukocyte function-associated molecule 1 alpha chain
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
