Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
The Rat Macrophage Inflammatory Protein-1 Beta (MIP1B) ELISA Kit is specifically designed for the precise measurement of MIP1B levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an excellent choice for a variety of research purposes.MIP1B, also known as CCL4, is a key chemokine involved in the regulation of inflammatory responses and immune cell recruitment. It plays a crucial role in various physiological and pathological processes, including immune defense, inflammation, and tissue repair.
Monitoring MIP1B levels can provide valuable insights into the immune response and help in the development of potential therapies for inflammatory conditions and immune-related disorders.Overall, the Rat Macrophage Inflammatory Protein-1 Beta (MIP1B) ELISA Kit is a valuable tool for researchers looking to study the role of MIP1B in immune regulation and inflammatory disorders in rat models.
Product Name:
Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Product Code:
RTDL00671
Size:
96 Assays
Target:
MIP1b
Synonyms:
CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
