SynonymsCCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
ReactivityRat
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
RTDL00656
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14844
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Rat Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
The Rat Monocyte Chemotactic Protein-1 (MCP-1) ELISA Kit is a reliable and accurate tool designed for the quantitative detection of MCP-1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.MCP-1 is a key chemokine involved in the recruitment and activation of monocytes, playing a critical role in inflammatory responses and immune regulation. Dysregulation of MCP-1 expression has been linked to various diseases, including cardiovascular diseases, neuroinflammatory disorders, and autoimmune conditions, making it a valuable biomarker for investigating these pathologies and potential therapeutic interventions.
With its user-friendly protocols and robust performance, the Rat MCP-1 ELISA Kit is an essential tool for researchers studying the complex mechanisms of MCP-1 and its implications in disease pathogenesis. Get reliable and reproducible data with this advanced ELISA kit from AssayGenie.
Product Name:
Rat Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
Product Code:
RTDL00656
Size:
96 Assays
Target:
MCP1
Synonyms:
CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
