Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit
The Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit is a powerful tool for the detection of MyD88 levels in rat samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for diverse research purposes.MyD88 is a key signaling adapter protein involved in the activation of innate immune responses and inflammatory pathways. Dysregulation of MyD88 has been linked to various diseases, including infections, autoimmune disorders, and cancer.
Therefore, measuring MyD88 levels in biological samples can provide valuable insights into the underlying mechanisms of these diseases and aid in the development of targeted therapeutic interventions.Overall, the Rat MyD88 ELISA Kit is an indispensable tool for researchers studying immune responses, inflammation, and related diseases in rat models. Its reliability and precision make it a valuable resource for advancing our understanding of MyD88 biology and its role in health and disease.
Product Name:
Rat Myeloid Differentiation Factor 88 (MyD88) ELISA Kit
Product Code:
RTDL00705
Size:
96 Assays
Target:
MyD88
Synonyms:
Myeloid Differentiation Primary Response Gene 88
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
