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Rat Myod1(Myoblast determination protein 1)ELISA kit
Key Features

Rat Myod1 (Myoblast determination protein 1) ELISA kit (AEFI00890)

Datasheet MSDS
SKU AEFI00890
Product Type ELISA Kit
Size 48T/96T
Reactivity Rat
Detection Method Sandwich ELISA, Double Antibody
Sensitivity 9.375 pg/mL
Range 15.625-1000 pg/mL
€649

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Description

system_update_altDatasheetsystem_update_altMSDS

Rat Myod1 (Myoblast determination protein 1) ELISA Kit

The Rat MYOD1 (Myoblast Determination Protein 1) ELISA Kit is specifically designed for the precise measurement of MYOD1 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research studies. MYOD1 is a key regulator of myoblast determination and differentiation, playing a crucial role in muscle development and regeneration. Dysregulation of MYOD1 is associated with various muscle disorders and diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

With its reliable performance and broad applicability, the Rat MYOD1 ELISA Kit is an essential tool for researchers studying muscle biology, muscular dystrophies, and muscle-related diseases. Trust in this kit to deliver precise and dependable measurements for your research needs.

Product Name:Rat Myod1 (Myoblast determination protein 1) ELISA Kit
SKU:AEFI00890
Reactivity:Rat
Assay Type:Sandwich ELISA, Double Antibody
Sensitivity:9.375 pg/mL
Range:15.625-1000 pg/mL
Sample Type:Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:2-8°C for 12 months.
Linearity:
Sample1:21:41:8
Serum (n = 5)87-97%88-102%85-96%
EDTA Plasma (n = 5)92-105%93-105%88-97%
Heparin Plasma (n = 5)89-101%84-99%81-100%
Recovery:
SampleRecovery Range (%)Average (%)
Serum (n = 5)92-10497
EDTA Plasma (n = 5)86-10594
Heparin Plasma (n = 5)85-10092
Assay Procedure

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample TypeProtocol
SerumAllow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
PlasmaCollect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue HomogenateHomogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture SupernatantCentrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell LysateLyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample TypesFor more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
ComponentQuantityStorage
48T96T
ELISA Microplate (Dismountable)8×68×12Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard1 vial2 vialPlace the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)60 ul120 ul2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)60 ul120 ul2-8°C (Avoid direct light)
TMB Substrate5 ml10 ml2-8°C (Avoid direct light)
Sample Dilution Buffer10 ml20 ml2-8°C
Antibody Dilution Buffer5 ml10 ml2-8°C
SABC Dilution Buffer5 ml10 ml2-8°C
Stop Solution5 ml10 ml2-8°C
Wash Buffer(25X)15 ml30 ml2-8°C
Plate Sealer3 pieces5 pieces-
Technical Manual1 copy1 copy-
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