Rat N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP) ELISA Kit
Based on the information provided in the URL, the Rat N-terminal Pro Atrial Natriuretic Peptide (NT-proANP) ELISA Kit offered by Assay Genie is a cutting-edge tool designed for the precise measurement of NT-proANP levels in rat serum, plasma, and cell culture supernatants. This kit boasts superior sensitivity and specificity, guaranteeing accurate and reproducible outcomes, rendering it well-suited for a variety of research endeavors.
NT-proANP is a pivotal protein associated with various physiological processes, including cardiovascular function and fluid balance regulation. Its measurement is crucial for understanding conditions like heart failure, hypertension, and renal diseases, hence serving as a vital biomarker for investigating these ailments and devising potential treatment strategies.
Product Name:
Rat N-Terminal Pro Atrial Natriuretic Peptide (NT-ProANP) ELISA Kit
Product Code:
RTDL00767
Size:
96 Assays
Target:
NT-ProANP
Synonyms:
NT-Pro-ANP, N-ANP
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
