The Rat Neuritin-1 (NRN1) ELISA Kit is specifically designed for the sensitive and accurate detection of Neuritin-1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit has been optimized for high specificity and precision, ensuring reliable and reproducible results for a variety of research applications.Neuritin-1, also known as CPG15, is a protein that plays a critical role in neuronal development and plasticity. It is involved in processes such as synaptogenesis, neurite outgrowth, and neuronal survival, making it a key player in nervous system function.
Dysregulation of Neuritin-1 has been linked to various neurological disorders, making it an important biomarker for investigating these conditions and potential therapeutic interventions.By utilizing the Rat Neuritin-1 (NRN1) ELISA Kit, researchers can accurately quantify Neuritin-1 levels in rat samples, allowing for a better understanding of its role in neurodevelopment and disease pathology. This reliable and sensitive assay is an invaluable tool for advancing research in the fields of neuroscience, neurobiology, and neurology.
Product Name:
Rat Neuritin 1 (NRN1) ELISA Kit
Product Code:
RTDL00763
Size:
96 Assays
Target:
NRN1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
