The Rat Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit is specifically designed for the precise quantification of NPY2R levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable tool for researchers in various fields.NPY2R is a crucial receptor involved in the regulation of neuropeptide Y, a key neurotransmitter that plays a role in various physiological processes such as feeding behavior, stress response, and cardiovascular function.
Dysregulation of NPY2R has been implicated in conditions such as obesity, anxiety disorders, and cardiovascular diseases, making it a valuable target for research and potential therapeutic interventions.Overall, the Rat Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit offers a powerful tool for studying the role of NPY2R in various biological processes and diseases, providing researchers with valuable insights for advancing their research and potential clinical applications.
Product Name:
Rat Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit
Product Code:
RTDL00755
Size:
96 Assays
Target:
NPY2R
Synonyms:
NPY-Y2 receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
