Rat NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like Protein 2) ELISA Kit
The Rat NFE2L2 (Nuclear Factor Erythroid-derived 2-like protein 2) ELISA Kit is specifically designed for the precise measurement of NFE2L2 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring the reliability and reproducibility of results for a variety of research applications.NFE2L2 is a critical transcription factor involved in oxidative stress response and regulation of antioxidant genes. Its dysregulation has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. By accurately quantifying NFE2L2 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.
Product Name:
Rat NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like Protein 2) ELISA Kit
SKU:
RTES00874
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
