The Rat NFKB Activating Protein (NKAP) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of NKAP levels in rat samples, including serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, ideal for various research applications.NKAP is a key protein that plays a critical role in activating the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a transcription factor that regulates genes involved in inflammation, immunity, and cell survival. Dysregulation of NF-κB signaling is implicated in various diseases, including cancer, inflammatory disorders, and autoimmune conditions, making NKAP a valuable biomarker for studying these pathologies and potential therapeutic targets.
Overall, the Rat NFKB Activating Protein (NKAP) ELISA Kit is a powerful tool for researchers studying NF-κB signaling pathways and investigating the role of NKAP in disease pathogenesis. With its high sensitivity and specificity, this kit provides accurate and quantitative measurements of NKAP levels, facilitating in-depth investigations and advancements in biomedical research.
Product Name:
Rat NFKB Activating Protein (NKAP) ELISA Kit
Product Code:
RTDL00733
Size:
96 Assays
Target:
NKAP
Synonyms:
NF Kappa B Activating Protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
