The Rat NRP1 (Neuropilin 1) ELISA Kit is a sophisticated diagnostic tool meticulously crafted for the precise quantification of Neuropilin 1 protein levels in diverse rat biological samples. Neuropilin 1 is a fundamental cell surface receptor involved in various cellular functions such as axon guidance, angiogenesis, and immune response modulation. Its intricate role as a co-receptor for multiple signaling pathways underscores its significance in various physiological and pathological processes. This ELISA kit empowers researchers with the capability to quantitatively measure NRP1 levels, providing valuable insights into its expression patterns, regulatory mechanisms, and functional implications in rat models. Accurate determination of Neuropilin 1 is essential for unraveling its diverse functions, from nervous system development to cancer progression, making it an indispensable tool for dissecting complex cellular interactions. Crafted with precision and adherence to rigorous quality standards, the Rat NRP1 ELISA Kit from Assay Genie ensures unparalleled sensitivity and specificity, guaranteeing dependable and reproducible outcomes. With its user-friendly design and high-performance characteristics, this kit is a preferred choice for researchers aiming to elucidate the intricate role of Neuropilin 1 in biological systems and disease pathogenesis.
Product Name:
Rat NRP1 (Neuropilin 1) ELISA Kit
SKU:
AEES00551
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
