The Rat PGA (Pepsinogen A) ELISA Kit is a highly specialized and sensitive assay designed for the precise measurement of pepsinogen levels in rat serum, plasma, and tissue samples. This kit offers unparalleled accuracy and reproducibility, making it an invaluable tool for researchers studying gastric health and function.Pepsinogen A is an important enzyme produced by the stomach that is involved in the digestion of proteins. Abnormal levels of pepsinogen A can be indicative of various gastric diseases, such as gastritis and peptic ulcers.
By accurately measuring pepsinogen A levels, researchers can gain valuable insights into the health of the gastrointestinal tract and potential disease mechanisms.With its high sensitivity and specificity, the Rat PGA ELISA Kit is a valuable resource for researchers looking to study gastric health, assess the efficacy of therapeutic interventions, and develop novel treatments for gastric diseases. Get your hands on this advanced assay kit today and unlock the potential of your research.
Product Name:
Rat Pepsinogen A (PGA) ELISA Kit
Product Code:
RTDL00825
Size:
96 Assays
Target:
PGA
Synonyms:
PGA5, PG-A, Pepsinogen 5 Group I, Pepsinogen-5, Pepsin A-5
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
