Rat Ppp1r15a (Protein phosphatase 1 regulatory subunit 15A) ELISA Kit
The Rat PPP1R15A (Protein Phosphatase 1 Regulatory Subunit 15A) ELISA Kit is a top-of-the-line assay designed for the precise quantification of PPP1R15A levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. PPP1R15A is a critical regulatory subunit of protein phosphatase 1, playing a key role in cellular stress responses and protein synthesis regulation.
Dysregulation of PPP1R15A has been implicated in various diseases including cancer, diabetes, and neurodegenerative disorders, highlighting its importance as a potential therapeutic target and biomarker. By utilizing the Rat PPP1R15A ELISA Kit, researchers can investigate the role of PPP1R15A in disease pathogenesis and explore its potential as a target for novel therapeutic interventions. Trust in this advanced ELISA kit for accurate and reliable results in your research endeavors.
Product Name:
Rat Ppp1r15a (Protein phosphatase 1 regulatory subunit 15A) ELISA Kit
SKU:
AEFI00901
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
46.875 pg/mL
Range:
78.125-5000 pg/mL
Sample Type:
Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-97%
85-96%
90-99%
EDTA Plasma (n = 5)
86-105%
91-98%
87-95%
Heparin Plasma (n = 5)
87-104%
86-97%
86-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-101
94
EDTA Plasma (n = 5)
94-104
99
Heparin Plasma (n = 5)
92-100
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
