Rat Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit
The Rat Prostaglandin Endoperoxide Synthase-1 (PTGS1) ELISA Kit is specifically designed for the accurate measurement of PTGS1 levels in rat serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, allowing for precise and reliable results that can be used in a variety of research applications.PTGS1, also known as cyclooxygenase-1 (COX-1), is an enzyme that plays a key role in the synthesis of prostaglandins, which are important mediators of inflammation and pain. Dysregulation of PTGS1 has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
By utilizing the Rat PTGS1 ELISA Kit, researchers can accurately quantify PTGS1 levels in rat samples, providing valuable insights into the role of this enzyme in inflammation and disease pathology. This kit is a valuable tool for advancing research in areas such as pharmacology, immunology, and molecular biology.
Product Name:
Rat Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
