Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit
The Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit is a highly precise and reliable assay designed for the quantitative detection of PDK1 levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PDK1 is a key regulator of the pyruvate dehydrogenase complex, playing a crucial role in the control of glucose metabolism and energy production. Dysregulation of PDK1 is implicated in various metabolic disorders, making it a valuable target for studying metabolic pathways and developing potential therapeutic interventions.
This ELISA kit provides researchers with a powerful tool for investigating the role of PDK1 in metabolic diseases, offering insights into its potential as a biomarker and therapeutic target. Trust in the accuracy and performance of the Rat PDK1 ELISA Kit for your research needs.
Product Name:
Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
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