Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
The Rat RBP4 (Retinol-Binding Protein 4) Plasma CLIA Kit is a reliable and accurate tool for measuring RBP4 levels in rat plasma samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for research applications.RBP4 is a key protein involved in retinol transport and metabolism, playing a critical role in various physiological processes including insulin resistance, diabetes, and obesity.
Monitoring RBP4 levels can provide valuable insights into metabolic disorders and potential therapeutic targets.With the Rat RBP4 Plasma CLIA Kit, researchers can effectively study the role of RBP4 in rat models and explore its significance in various metabolic conditions. Trust in this kit for dependable results and expedite your research in the field of metabolic disorders.
Product Name:
Rat RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit
SKU:
RTES00501
Target:
Rat RBP4 (Retinol Binding Protein 4, Plasma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RBP4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat RBP4. You can calculate the concentration of Rat RBP4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
85-98
97-112
Average (%)
95
90
103
1:4
Range (%)
89-103
99-110
88-101
Average (%)
96
104
94
1:8
Range (%)
87-99
101-113
100-116
Average (%)
93
108
107
1:16
Range (%)
99-114
85-100
93-108
Average (%)
106
92
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
101-116
106
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.69
8.21
0.93
2.65
8.4
Standard deviation
0.09
0.2
0.82
0.08
0.26
0.88
C V (%)
9.78
7.43
9.99
8.6
9.81
10.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat RBP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat RBP4 in samples. No significant cross-reactivity or interference between Rat RBP4 and analogues was observed.
