Rat Receptor Interacting Serine Threonine Kinase 1 (RIPK1) ELISA Kit
The Rat Receptor Interacting Serine/Threonine Kinase 1 (RIPK1) ELISA Kit is specifically designed for the quantitative detection of RIPK1 levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it perfect for a variety of research applications.RIPK1 is a key regulator of inflammatory and necroptotic cell death pathways, playing a crucial role in various disease processes such as inflammation, cancer, and neurodegenerative disorders.
By measuring RIPK1 levels, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially identify new targets for therapeutic intervention. Overall, the Rat RIPK1 ELISA Kit provides researchers with a powerful tool for studying the role of RIPK1 in disease pathogenesis and evaluating its potential as a therapeutic target.
Product Name:
Rat Receptor Interacting Serine Threonine Kinase 1 (RIPK1) ELISA Kit
Product Code:
RTDL00905
Size:
96 Assays
Target:
RIPK1
Synonyms:
RIP, Receptor-Interacting Protein, Cell death protein RIP, Serine/threonine-protein kinase RIP
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
