The Rat S100A4 ELISA Kit is specifically designed to detect and quantify levels of S100A4 protein in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research experiments. S100A4 is a multi-functional protein that plays a crucial role in cell migration, proliferation, and invasion. Its overexpression has been linked to cancer metastasis and tumor progression, making it a valuable biomarker for studying cancer development and progression.
Additionally, S100A4 is involved in inflammatory processes and tissue regeneration, highlighting its importance in various biological pathways. With its advanced technology and easy-to-follow protocol, the Rat S100A4 ELISA Kit is an essential tool for researchers studying S100A4 protein expression and function in rat models. Whether investigating cancer biology, inflammation, or tissue repair, this kit provides accurate and precise measurements to support your research objectives.
Product Name:
Rat S100A4 ELISA Kit
SKU:
RTFI00378
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-101%
85-105%
85-101%
EDTA Plasma (n = 5)
82-100%
83-100%
83-95%
Heparin Plasma (n = 5)
80-97%
81-95%
83-95%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-98
92
EDTA Plasma (n = 5)
88-104
98
Heparin Plasma (n = 5)
86-100
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
