Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
The Rat TCC/C5b-9 Terminal Complement Complex (C5b-9) ELISA Kit is designed for the precise measurement of C5b-9 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The C5b-9 terminal complement complex is a crucial component of the complement system, playing a key role in immune response and inflammation. Dysregulation of the complement system has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and infectious diseases, making the measurement of C5b-9 levels vital for understanding disease pathogenesis and developing potential therapeutics. With its advanced technology and high-quality components, the Rat TCC/C5b-9 Terminal Complement Complex ELISA Kit provides researchers with a valuable tool for studying the complement system and its involvement in health and disease.
Product Name:
Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
SKU:
RTES00792
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
