The Rat Transferrin (TF) ELISA Kit is a sophisticated assay designed to accurately quantify the levels of transferrin in a variety of biological samples derived from rat models. Transferrin, a glycoprotein crucial for iron transport and homeostasis, plays a pivotal role in various physiological processes, including iron binding, delivery, and regulation, making it a key player in iron metabolism. This ELISA kit offers researchers a reliable and precise method for assessing transferrin levels, providing valuable insights into iron transport mechanisms and iron-related disorders. By enabling quantitative analysis of transferrin with exceptional sensitivity and specificity, researchers can deepen their understanding of iron metabolism, anemia, and other iron-related conditions in rat models. Manufactured under strict quality control standards, the Rat Transferrin ELISA Kit guarantees robust performance and accurate results, making it an indispensable tool for research studies focusing on iron homeostasis, transferrin biology, and iron-related disorders in rat models.
Product Name:
Rat TF (Transferrin) ELISA Kit
SKU:
AEES00542
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
