The Rat Tight Junction Protein 1 (TJP1) ELISA Kit is a powerful tool for the precise measurement of TJP1 levels in rat samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides consistent and accurate results, making it a valuable asset for various research studies.TJP1, also known as ZO-1, is a key component of tight junctions, playing a crucial role in maintaining cell barrier function and integrity.
Dysregulation of TJP1 has been implicated in various diseases, including inflammatory bowel disease, cancer, and neurological disorders, underscoring its importance as a biomarker for disease progression and therapeutic targeting.Overall, the Rat TJP1 ELISA Kit offers researchers a reliable and convenient method for studying the role of TJP1 in health and disease, paving the way for potential therapeutic interventions and improved patient outcomes.
Product Name:
Rat Tight Junction Protein 1 (TJP1) ELISA Kit
Product Code:
RTDL01033
Size:
96 Assays
Synonyms:
ZO1, Zona Occludens 1, Zona occludens protein 1, Zonula occludens protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
