The Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is designed for the quantitative detection of TNF-α levels in rat serum, plasma, cell supernatant, lysates, and other biological samples. TNF-α is a 17 kDa pleiotropic pro-inflammatory cytokine primarily produced by activated macrophages, monocytes, T lymphocytes, and natural killer cells. It is initially synthesized as a 26 kDa transmembrane precursor that is cleaved by TNF-α converting enzyme (TACE/ADAM17) to release the soluble bioactive form, which signals through TNFR1 and TNFR2 to regulate immune cell activation, apoptosis, NF-κB signaling, and acute-phase responses. TNF-α plays a central role in host defense against infection but is also a key driver of chronic inflammatory and autoimmune diseases including rheumatoid arthritis, inflammatory bowel disease, psoriasis, septic shock, cachexia, insulin resistance, and various cancers, making it one of the most studied cytokines and a major therapeutic target. Accurate measurement of TNF-α is essential for understanding inflammatory and immune disease mechanisms and for developing targeted therapeutic strategies. Assay Genie's Rat TNF-α ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and translational applications. Trust Assay Genie's Rat TNF-α ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
SKU:
AEES05546
Size:
96T
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
~3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Recovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the wells, allowing the target to bind to the immobilized antibody. After incubation, a biotinylated detection antibody specific for Rat TNF-α is added, which binds specifically to the captured target. Following a wash step to remove excess detection antibody, HRP-conjugated Streptavidin is introduced, forming a biotin-streptavidin-HRP complex. After a second washing step, Substrate Reagent is added to initiate a colorimetric reaction catalyzed by HRP. The reaction produces a blue product that turns yellow upon addition of the acidic Stop Solution. The optical density (OD) is measured at 450 nm using a microplate reader. The OD450 value is directly proportional to the concentration of TNF-α in the sample, which can be determined by referencing a standard curve.
